The goal of the comprehensive reform of agricultural water prices is to establish an incentive mechanism and take economic measures to promote agricultural water use. Jinan District, Lu'an City, as a national advanced area for comprehensive reform of agricultural water prices, we proposed the "seven-step working method" for comprehensive agricultural water price reform; we improved the management organization system of "irrigation area + water cooperatives + water users", and cultivated a national-level for "farmers water cooperation demonstration organization" to form specific operation methods, and its practical experience can provide a reference for relevant regions.